Consistent with RIG-I activation, we observed increasedlevels of pIRF3 in the TME (Figure 4B) and expression of ISGs and proinflammatory cytokines(Figure 4C) in micetreated with SLR-LNPs, but not empty LNPs or cSLR-LNP formulations.We also observed a significant increase in the number of tumor-infiltratingCD4+ and CD8+ T cells in response to SLR-LNPtreatment, but no significant differences in the number of NK cells,dendritic cells, macrophages, or MDSCs (Figure 4D). Here, CD8A is linked to neoplasm.