NLRC4 and neoplasm: To address the clinical consequences of NLRC4 expression in tumor tissues, the patients’ cohort was stratified based on protein expression levels of NLRC4, IL-1β, or IL-18, as high versus low expression for each marker (above/below median; see Supplemental Figure 5 and Methods), either in the colon epithelium (within the cytokeratin mask) or in the stroma (outside the cytokeratin mask) (see Methods).