TIMMDC1 and esophageal squamous cell carcinoma: To investigate the molecular function of TIMMDC1 in ESCC, 2 specific short hairpin TIMMDC1 (shTIMMDC1) sequences were designed to knock down TIMMDC1 in the KYSE30 and KYSE450 cell lines, which express relatively high levels of TIMMDC1 (Supplemental Figure 4B).