Using validated specific antibodies (Supplemental Figure 1; supplemental material available online with this article; https://doi.org/10.1172/jci.insight.164191DS1), we observed NKX2-5 expression in the pulmonary vasculature of patients with hypertension (Figure 1) as well as in diseased carotid, coronary, and peripheral arteries (Supplemental Figure 2), where it coincides with ACTA2 expression in VSMCs (Supplemental Figure 2, F and G). This evidence concerns the gene NKX2-5 and Hypertension.