The percentages of CD45+ cells (32.46% vs. 3.096%), macrophage (4.536% vs. 1.312%), and M1 macrophage (4.359% vs. 0.999%) were higher in samples from non-metastatic osteosarcoma primary lesions than those in metastatic osteosarcoma primary lesions (Fig. 6b). The gene discussed is PTPRC; the disease is osteosarcoma.