To verified the findings based on scRNA-seq data, IHC was used to detect PDGFD and PDGFRB expression in non-metastatic and metastatic osteosarcoma primary lesions, and found that PDGFD was mainly expressed in osteosarcoma cells, while the PDGFRB expression was observed in both osteosarcoma cells and stromal cells. Here, PDGFRB is linked to osteosarcoma.