In primary cells derived from non-metastatic osteosarcoma, the number of invaded cells per field of shPDGFD group was higher than that of shControl group (61.8 ± 9.13 vs. 6 ± 3.56, p < 0.0001, Fig. 8a, c), and PDGFD OE group had less invaded cells per field than control group (7.4 ± 3.26 vs. 15 ± 4.43, p = 0.0245, Fig. 8a, d). Here, PDGFD is linked to osteosarcoma.