We found high levels of CCL-20, CXCL1, EGF, GCSF, GMCSF, IL6, OPN, PDGF-AA, and PTX3 in the culture media of TKTB34-RAS, which caused severe skeletal muscle defects, compared with the TKTB34-PIK3CA tumor line. This evidence concerns the gene SPP1 and neoplasm.