After a prolonged exposure, the results from this study showed that DEP seems to share the same vasorelaxant mechanism as estrogens in the HUA, through the NO/sGC/cGMP/PKG signaling pathway, and seems to interfere with the L-type Ca2+ channels, diminishing NIF vasorelaxant effects contributing to hypertension. The gene discussed is PRKG1; the disease is hypertensive disorder.