RNAi experiments were conducted in the HCT116:Ki-67-AID (cell line we have generated), the original HCT116 carrying Ki-67 wt alleles, an HCT116 cell line where the MHL1 wt gene has been re-introduced (to note that HCT116 have a mutation in the DNA repair enzyme MHL1), SW840, a p53 negative colorectal cancer (HCT116 are p53 wt), HeLa and an immortalised non-cancerous cell line hTERT-RPE1. The gene discussed is MKI67; the disease is colorectal cancer.