Similarly, compared with mice in the POCD + OI group, the expression of IL-1β (36.37 ± 7.809 vs 20.26 ± 5.713 pg/mg, P = 0.0133) and IL-6 (45.82 ± 10.38 vs 23.48 ± 7.269 pg/mg, P = 0.0111) were increased in mice of POCD + OI + ML385 group 24 h after surgery, and immunofluorescence staining results showed a significant increase in microglia and astrocytes labeled by Iba1 (87.41 ± 11.70 vs 62.61 ± 9.535 cells, P = 0.0246) and GFAP (50.80 ± 12.80 vs 27.93 ± 6.269 cells, P = 0.0312), respectively, in the POCD + OI + ML385 group compared to the POCD + OI group. The gene discussed is IL1B; the disease is osteogenesis imperfecta.