AQP1 and triple-negative breast carcinoma: To this end, we used lentiviral infection to express Aqp1 from a strong constitutively active promoter (EF1α) in five cell lines: HT22 (hippocampal), J774A.1 (macrophage), Jurkat (T lymphocyte), MDA-MB-231 (triple-negative breast cancer), and MIN6 (insulinoma).