In order to further examine the effect in clinical application, the immunohistochemistry (IHC) technique was used to evaluate the expression levels of the five differentially expressed genes (TRIM36, CYP26B1, PAGE1, CDK5R2, and DCAF8L1) in 90 HCC tissues and 90 para-carcinoma tissues on a tissue microarray. The gene discussed is TRIM36; the disease is hepatocellular carcinoma.