ERβ knockdown blocked the effect of 25-HC on LAC proliferation, migration and invasion.27-hydroxycholesterol, as a structural analogue of 25-HC, has been identified as an ERβ-selective regulator (SERM) that dose-dependently inhibited ERβ transcriptional activity in aortic endothelial cells and breast cancer cells [22], but dose-dependently elevated ERβ transcriptional activity in the hepatocellular carcinoma cells and colon cancer cells [23]. The gene discussed is ESR2; the disease is hepatocellular carcinoma.