In this study, we investigated the role of PAFAH1B3 in promoting the proliferation, invasion, migration and metastasis of pancreatic cancer cells in vitro and in vivo and explored the mechanism by which PAFAH1B3 mediates the ability of KLF9 to inhibit the proliferation, invasion and metastasis of pancreatic cancer cells. The gene discussed is PAFAH1B3; the disease is pancreatic neoplasm.