SCAI and cancer: Similar to blocking Ago2/CAV1 interaction with P2 peptides, blocking Ago2/CAV1 interaction with IAA in A549Ago2-KO/HA-AID-Ago2Wt/HA-Ago2∆ cells decreased miR-3613-3p, increased SCAI mRNAs (Fig. EV3Di,Dii), selectively impaired the suppression of miR-3613-3p mimics on SCAI mRNAs (Fig. EV3E), increase resistance to trypsin disassociation (Fig. EV3F), decrease anoikis resistance (Fig. EV3G), attenuate tumorsphere formation (Fig. EV3H), and suppress invasion (Fig. EV3I) of cancer cells.