Corresponding to the miRNA expression in cancer cells, the elevated levels of miRNA-3613-3p were detected in plasma EVs of A549Ago2-KO/HA-Ago2Wt tumor-bearing mice, and disruption of Ago2/CAV1 interaction decreased EV-mediated miR-3613-3p release in plasma EV of A549Ago2-KO/HA-Ago2∆ tumor-bearing mice. This evidence concerns the gene AGO2 and neoplasm.