AGO2 and cancer: To avoid this scenario, HA-Ago2 (Fig. EV2D, HA-Ago2Wt) and CBM-deleted HA-Ago2 (Fig. EV2D, HA-Ago2∆) were expressed in Ago2-knockout A549 cancer cells, in which the Ago2 gene was deleted using a CRISPR/Cas9 system (Fig. EV2D, A549Ago2-KO).