AICDA and cancer: In A549Ago2-KO/HA-AID-Ago2Wt/HA-Ago2∆ cells treated with IAA, which only expressed HA-Ago2∆, CAV1 was not precipitated with HA-Ago2∆ by anti-HA antibodies, indicating that Ago2/CAV1 interaction was blocked in IAA-treated cancer cells with an auxin-inducible degron system (Fig. EV3Ci,Cii).