In this study, we explored the Ago2/CAV1 interaction in cancer cells (i.e., A549 lung cancer cells, H1299 lung cancer cells, BxPC-3 pancreatic cancer cells, HCC1806 breast cancer cells, and 104-S, C4-2, and R-1 prostate cancer cells) and normal cells (i.e., BEAS-2B human lung epithelial cells, HPNE human pancreatic epithelial cells, and HMLE human mammary epithelial cells). Here, AGO2 is linked to familial pancreatic carcinoma.