And treatment with NAC for a duration of 1 week starting at 1 week after diabetes induction significantly enhanced post-ischemic levels of Slc7a11, Gpx4 (Fig. 3C) and GSH (Fig. 3F), decreased lipid peroxidation evidenced as reduction in MDA (Fig. 3C) and 15-F2t-Isoprostane (Fig. 3D) and reduced levels of labile iron (Fig. 3E), and attenuated post-ischemic ferroptosis and myocardial cellular damage (Fig. 3B). This evidence concerns the gene GPX4 and diabetes mellitus.