Several adjunctive therapies have been suggested to enhance the efficacy of cryosurgery, namely, chemical adjuvants such as cancer chemotherapeutic agents (Feeney and Yeh 1998; Kim et al. 2017; Raymond and DeVries 1972), AFP I (Bagis et al. 2008; Feeney and Yeh 1998; Leygonie et al. 2012; Rubinsky et al. 1994), amino acid adjuvants such as glycine (Wang et al. 2008) and tumor necrosis factor alpha (TNF-α) have all been investigated for this purpose (Goel et al. 2007). This evidence concerns the gene TNF and cancer.