Moreover, steatohepatitis with fibrosis was successfully established in WD-fed/CCl4-treated model as evidenced by H&E and SR staining (Figure S8A), hepatic Hyp content (Figure S8B), as well as Col-I, Col-III, Col-IV, α-SMA and TGF-β1 expressions (Figure S8C, S8F), which were similar to the report 17 and consistent in patients with NASH 8, 18. This evidence concerns the gene ACTA1 and Wilson disease.