The TLR4/MyD88/TRIF/NF-κB signaling pathway and ACLY activity were inhibited in OVA-induced MH-S cells with knockdown of S100A8 or S100A9 and allergic asthma mice, suggesting that knockdown of S100A8 or S100A9 inhibits glycolysis by suppressing the TLR4/MyD88/TRIF/NF-κB signaling pathway. Here, S100A9 is linked to allergic asthma.