MGMT and glioblastoma: This method enables real-time, single-molecule sequencing without the need for amplification, potentially offering advantages in longer DNA fragment sequencing and rapid data generation.94-96 A proof-of-concept study on 45 patients with glioblastoma showed that nanopore sequencing can accurately categorize MGMT methylation profiles, yielding precision akin to the EPIC array method and resolution markedly better than next-generation sequencing panel sequencing.97