While most GFP-labeled adipocyte nuclei robustly expressed PPARγ proteins under typical conditions, a significant proportion of adipocyte nuclei from eWAT in HFD-fed mice lacked PPARγ proteins (Figure 6E, 6F), confirming the presence of PPARγ-deficient Ad6 adipocytes in eWAT during obesity. This evidence concerns the gene PPARG and obesity due to melanocortin 4 receptor deficiency.