In order to assess the potential involvement of the TGF-β signaling pathway in the progression of ALS in this zebrafish model, we knocked down the tgfb1a by co-microinjecting hSOD1G93A mRNA along with an antisense morpholino (MO) targeting tgfb1a mRNA (CAGCACCAAGCAAACCAACCTCATA; GeneTools) at the one cell stage (Supplementary File 1). Here, TGFB1 is linked to amyotrophic lateral sclerosis.