To gain more proof to certify the biological function of USP19‐MGMT axis in GBM cells chemo‐resistance in vivo, we employed shRNA to specifically knockdown USP19 in T98G cells, and nude mice were subcutaneously implanted with T98G USP19 depletion cells and control (Ctrl) cells. The gene discussed is MGMT; the disease is glioblastoma.