Notably, examination at various time points (24, 48, 72, and 96 h) revealed that miR-100-5p exerted inhibitory effects on RA-FLS proliferation under the stimulation of IL-1β or TNF-α, particularly evident at 72- and 96-hour post-stimulation (Fig. 5C). The gene discussed is IL1B; the disease is rheumatoid arthritis.