ANGPTL4 and colorectal carcinoma: To elucidate the influence of ANGPTL4 on the behavior of CRC cells, we transfected SW480 and RKO cells with ANGPTL4-overexpression plasmid or ANGPTL4-targeting shRNA, which were validated by qRT-PCR and western blot (Fig. 4A, B; Supplementary Fig. S3A, B).