Finally, a plausible identification revealed the upregulation of the ferroptosis regulator ZFP36 and GPX4 and the downregulation of the ferroptosis regulator CD44 and TRF1 in DKD through the utilization of IHC in DKD tissues and db/db spontaneous type 2 diabetes mouse model and WB in mouse podocyte MPC5 and mesangial SV40-MES-13 cells under HG conditions. Here, GPX4 is linked to type 2 diabetes mellitus.