We observed heterogeneous cells within the LCAs, including the EpCAM+ tumor cells, α-SMA+ CAFs and CD45+/CD3+ immune cells (Fig. 3c, d, Supplementary Fig. 4c–e), which indicated that LCAs could recapitulate a certain tumor microenvironment of the parental tumors. The gene discussed is ACTA1; the disease is neoplasm.