Then, low-dose ultrasound irradiation of the tumor site induces ROS generation, penetrates the cell membrane to activate caspase-3, and releases HYD to increase the expression of GSDME, a pyroptosis substrate specifically for ROS mediated caspase-3 cleavage, which synergistically leads to the pyroptosis of cancer cells (Fig. 1). Here, GSDME is linked to cancer.