Moreover, after taking into account the subgroup characterized by NT-proBNP ≥ 9531 pg/mL, B-lines ≥ 13, and intercostal spaces of pleural effusion ≥ 1, the multivariable analysis reported a further significant higher risk of HFrEF, almost up to five times, even after adjustment for age, sex and renal function. The gene discussed is NPPB; the disease is Pleural effusion.