CCK8 assay, colony formation assay and EdU assay demonstrated an enhanced growth ability of RBE and HuCCT1 cells treated with circSLCO1B3 overexpressed plasmid compared with ICC cells treated with mock plasmid, then the increasing trend was suppressed by ablating HOXC8 (Fig. 5e, f, g, h, i, j, l, m). The gene discussed is HOXC8; the disease is intrahepatic cholangiocarcinoma.