Collectively, this work shows that CRD-dependent galectin-3 binding interactions are implicated in TGF-β1 signaling in human lung fibroblasts and that the inhibition of these interactions can modulate TGFβ-dependent signaling and release of fibrogenic mediators in PCLuS from IPF patients. This evidence concerns the gene TGFB1 and idiopathic pulmonary fibrosis.