To better understand the structural and functional impact of heterozygous missense variant on protein and the potential contribution to the etiology of hearing loss, the human TNC protein (951 amino acids) and TBC1D24 protein (524 amino acids) were modeled using AlphaFold online prediction program (https://alphafold.ebi.ac.uk/, Fig. 3A) and SWISS Model (http://swissmodel.expasy.org/, Fig. 3B), The model covered the target sequence of TNC and TBC1D24 protein. Here, TBC1D24 is linked to hearing loss disorder.