PRNP and Creutzfeldt Jacob disease: To assess the sensitivity of sfRT-QuIC with a variety of PrP prion strains, end-point dilution analyses were performed on brain homogenates (Fig 2A) from cases of human sporadic (s) CJD (MM1 subtype), genetic (g) CJD (E200K mutant), Gerstmann-Sträussler-Scheinker disease (GSS; P102L mutant); ovine classical scrapie (ARQ/ARQ PrP genotype), and deer CWD (Fig 5).