Remarkably, ACO1 knockdown only inhibited proliferation of the high-MEMO1 cells, but not MEMO1 knockout breast cancer cells (Figure 2D), while SLC25A28 (Figure 2E, Figure 2—figure supplement 2) and PLOD1 (Figure 2F, Figure 2—figure supplement 3) knockdowns showed some effect in all cell lines, but a stronger inhibition in the high-MEMO1 cells than in MEMO1 knockout. Here, ACO1 is linked to breast cancer.