To confirm the functional link between iron homeostasis and MEMO1 experimentally, we generated clonal MEMO1 knockdowns and knockouts in MDA-MB-231 triple negative breast cancer and A-375 melanoma cell lines using the CRISPR-Cas9 technology and compared the effects of shRNA knockdown of selected genes involved in iron homeostasis on the proliferation rates of the cells with high (parental), low (knockdown), and no (knockout) MEMO1 expression. The gene discussed is MEMO1; the disease is melanoma.