By employing PTBP1 knockdown in glioblastoma cells, the study observed decreased EGFR protein levels, attenuated EGFR phosphorylation, altered EGFR localization within endosomes, and compromised ERK1/2 activation, and this conclusion was corroborated by concurrent PTBP1 and ANXA7-I1 knockdown, affirming PTBP1’s role in ANXA7-I1 splicing regulation to enhance EGFR signaling and promote tumor proliferation in glioblastoma cells. Here, PTBP1 is linked to neoplasm.