MYC and osteosarcoma: We used the CCLE database to examine the changes in the copy number and mRNA expression of the MYC gene in osteosarcoma cell lines (Figures 2B,C), and selected SJSA, SAOS2, 143B, U2OS, and HOS - five types of human osteosarcoma cell lines - to detect MYC protein expression levels (Figure 2D).