We next complemented our flow cytometry findings with multiplex immunohistochemistry (mIHC) on 30 GEACs (n=14 EAC; n=9 GEJAC; n=7 GAC (Figures 3A, B; Supplementary Tables 12, 13) and confirmed that EACs had lower median densities of CD3+ cells in general (14.0 vs 167.0 cells/mm2 p=0.005) and both CD4 cells (11.5 vs 130.0 cells/mm2, p=0.006) and CD8 cells (0.0 vs 37.0, p<0.001) compared to GACs in tumor and even more significant in stroma (Figure 3C). The gene discussed is CD8A; the disease is neoplasm.