After spleen macrophage depletion, 7-mix and mix-sup decreased the levels of M1-associated pro-inflammatory cytokines (iNOS, IL-1β, IL-6, TNF-α, and IL-23) and increased the levels of M2-related anti-inflammatory cytokines (CD206, TGF-β, and IL-4) in colon tissues of CLD-treated colitic mice (Fig. 6A). Here, TNF is linked to congenital secretory chloride diarrhea 1.