Furthermore, using the expression of TTN-AS1 in normal tissues as a control, the ROC curve of TTN-AS1 in cholangiocarcinoma tissues was plotted, resulting in an AUC of 0.637 with a 95% confidence interval of 0.501–0.773, a sensitivity of 68.75%, and a specificity of 69.13% (Fig. 5F). This evidence concerns the gene TTN and cholangiocarcinoma.