To further explore the effects of TTN-AS1 depletion, overexpression, and CTC-derived exosomes containing PTENP1 in CCA in vivo, sh-TTN-AS1-Exo, TTN-AS1-Exo, and CTCs-Exo were co-cultured with Huh28 cells and then injected into nude mice. The gene discussed is PTENP1; the disease is cholangiocarcinoma.