To corroborate the tumor-origin of TP53 mutations classified as non-hematopoietic in origin, we categorized their VAF dynamics in cfDNA during carboplatin treatment as increasing (VAF increase by factor 10), decreasing (VAF decrease by factor 10), or stable (all others), which correlated with radiographic response to carboplatin, PFS, and OS (Fig. 3c, d). Here, TP53 is linked to neoplasm.