RB1 and neurodegenerative disease: To determine the relationship between RB1 mutations and neurodegenerative disease, we calculated the RB1 mutation rates of PD (189 blood samples), AD (33 blood samples), HD (69 blood samples), and ALS (75 blood samples) using DNA sequence data from Guangzhou KingMed Diagnostics Group Co., Ltd. The results showed that the frequency of RB1 mutations in the coding region of the protein and RNA splicing was 2.64% in PD, 3.03% in AD, 2.85% in HD, and 2.66% in ALS (Fig. 1A and Table S1).