This is important because when comparing the adequacy of PD-L1 yields between EBUS-TBNA obtained specimens and surgically resected tumor biopsies at two separate cutoffs (≥ 1% and ≥ 50%), a significant decrease in the positive predictive value and sensitivities associated with a PD-L1 expression cutoff of ≥ 50% in EBUS-TBNA samples were observed. The gene discussed is CD274; the disease is neoplasm.