Similarly, the proliferative capacity of tumour explants was maintained for the length of the culture period, as shown by positive IHC staining for two independent proliferation markers, Ki67 and PHH3 (Fig 2), although expression of these markers is lower in cultured explants compared to uncultured tissue (Fig 2 and S1–S3 Figs). The gene discussed is MKI67; the disease is neoplasm.