VDJ gene usage analysis showed that certain VJ sequences, such as TRAV13-1, TRAV9-2, TRBV12-3 and TRAJ-49 (p < 0.05) were used more frequently in APS-1 patients compared to healthy controls (Figures 3A, 3B, and S8–S10). This evidence concerns the gene TRBV12-3 and autoimmune polyendocrine syndrome type 1.