We performed an association analysis of differential lipid metabolites and clinical indicators between the PCOS and CON groups (Fig. 3). The results were presented by hierarchical clustering heatmap: in the PCOS versus CON group,the concentrations TAG,DAG,PE,PC and HexCer-AP were found to be positively associated with TT,AMH, No. of oocytes retrieved, MII oocytes, and fertilization (P < 0.05). Here, AMH is linked to polycystic ovary syndrome.