Previous reports suggested that tumor-targeted therapy induces caspase-3 activation34 which can subsequently lead to pyroptosis initiated by caspase-3-mediated gasdermin E (GSDME) cleavage in response to various apoptotic stimuli.35 36 Indeed, we could confirm that BRAFi treatment of D4M melanoma cells in vitro induced caspase-3 cleavage as detected by capillary-based immmunoblotting with the JESS system, resulting in the cleavage of GSDME. This evidence concerns the gene CASP3 and melanoma.