TGFB1 and Hyperglycemia: Because hyperglycemia activates the TGFβ response34,35 and we demonstrated that Set7KO regulates key renal cell clusters, we stimulated human podocytes, glomerular endothelial cells, macrophage monocytes, and proximal tubular cells with PFI-2 before high-glucose and TGFβ1 stimulation (Figure 5F).