In the case of CD8+ pre-infection, the 4RVMHCII, and 6RVMHCII showed increased IFN-γ+ subpopulation compared to unstimulated samples, but after challenging, only the 4RVMHCII peptide showed a significant increase in IFN-γ+ subpopulation (Figure 11). This evidence concerns the gene CD8A and infection.