CD274 and breast cancer: PD-1 and PD-L1 expression were also associated with higher density of TILs and TLSs, in addition TILs density, TLSs and PD-L1 expression were associated with more aggressive tumor characteristics (90), on the basis of this study, researchers found a positive correlation between the expression of immune checkpoint molecules and baseline TILs and TLSs suggesting that assessing these parameters in breast cancer patients may identify immunomodulatory therapies in tumors that are responsive to them (91).