NLRP3 and major depressive disorder: We suspected that the non-significant increase in serum NLRP3 in adolescent depression patients may need to be considered the following factors: (1) the main location of the NLRP3 is intracellular, the inflammation in adolescents might not be severe enough to induce significant cellular damage for enhanced NLRP3 exposure or secretion; (2) Other psychiatric pathology studies revealed differences when examining NLRP3 in monocellucur blood cells via Western blot or Polymerase Chain Reaction, the method used in our study might not be sensitive to detect the NLRP3 expression.