Compared with WT or KO+/− mice injected with AAV-NC (WT-NC or KO+/−-NC), KO mice injected with AAV-NC (KO-NC) were significantly aggravated from 16-week HFD-induced hepatic steatosis, as shown by increased liver weights, the ratio of liver to body weight, ALT, AST and blood lipid levels, such as triglyceride (TG) and cholesterol (CHO) (Fig. 1j–o). This evidence concerns the gene GPT and fatty liver disease.